Electron Tomography and Synapse Study
نویسندگان
چکیده
Electron tomography (ET) is a useful tool to investigate threedimensional (3D) details based on virtual slices of relative thick specimen. A single electron transmission microscopic image is to ET what single X-ray image is to computed X-ray tomography (Rhyu & Park, 2008). As computed X-ray tomographic image shows cross sectional information of the body without real sectioning in hospital, we can get virtual cross sectional image of relative thick specimen up to resolution 2.4 Å recently (Scout et al., 2012). Not only technical upgrade of ET, but sophisticated analyses of various specimens are main interest of electron tomographic studies (Frey et al., 2006; Mun et al., 2008; Jou et al., 2013). By using relative thick specimen and very thin virtual slice up to 1 nm, ET is a useful tool dissecting very small structures and relatively large structures at the same time. PRINCIPLES OF ELECTRON TOMOGRAPHY
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